目的 考察通体结香技术产沉香(通体香)提取物用于保健食品研发剂量下(生药量0.60 g·d-1),对SD大鼠怀孕期间的致畸和致突变毒性。方法 试验通过对妊娠6~15 d的大鼠进行45、105、150 mg·kg-1通体香提取物灌胃,观察并记录孕鼠活动情况,测量并记录孕鼠体重,妊娠20 d后处死大鼠,解剖并记录观察胎仔存活率、吸收数等,并对胎仔进行解剖观察,记录胎仔畸形类型和数量。结果 通体香提取物45、105、150 mg·kg-1·bw-1 3个剂量组喂养孕鼠,对孕鼠体重无影响,对胎仔黄体数、着床数、活胎数、吸收胎数和死胎数等于对照组相比均无显著性差异(P>0.05),对胎仔的畸变类型和数量均无影响,与对照相比无显著性差异(P>0.05)。结论 通体香提取物在实验剂量下对孕期大鼠及胎仔无致畸和致突变毒性。
Abstract
OBJECTIVE To research the teratogenic and mutagenic toxicity of agarwood extracts produced by agar-wit technique for functional food development (slices dose 0.60 g·d-1). METHODS Pregnant rats of 6 to 15 d were given with agarwood extracts at 45, 105, 150 mg·kg-1 by gastric perfusion directly every day, then killed and anatomized after being pregnant for 20 d. During the whole course of treatment, the activity and body weight of pregnant rats, fetal survival rate, absorption number, malformation type and number of fetus were observed and recorded. RESULTS The agarwood extracts had no effect on the body weight of pregnant rats at 45, 105, 150 mg·kg-1 dosages, and the numbers of implantation, live fetuses, fetuses and stillbirths of fetus, malformation type and number had no significant difference compared with the control group (P>0.05). CONCLUSION Agarwood extracts have no teratogenic and mutagenic toxicity for rat fetuses.
关键词
通体结香技术 /
白木香 /
沉香 /
致畸 /
致突变
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Key words
agar-wit /
Aquilaria sinensis /
agarwood /
teratogenicity /
mutagenicity
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中图分类号：
R965
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参考文献
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脚注
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基金
国家重点研发计划项目资助(2018YFC1706400);中组部“万人计划”项目资助(99950534);中国医学科学院医学与健康科技创新工程项目资助(2016-I2M-2-003)
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